碧芯生物新品—24重可在Luminex200使用的二代羧基磁性熒光編碼微球-碧芯生物科技（海南?。┯邢薰?/title> <link type="text/css" rel="stylesheet" href="/skins/535403/css/style.css" /> <link rel="stylesheet" type="text/css" href="/skins/535403/css/swiper.min.css"> <script src="/skins/535403/js/jqueryNew.min.js"></script> <script src="/skins/535403/js/swiper.min.js"></script>  <script language="javascript" type="text/javascript"> var nav= '4'; </script>  <link rel="shortcut icon" href="/Skins/535403/favicon.ico" /> <script type="application/ld+json"> { "@context": "https://ziyuan.baidu.com/contexts/cambrian.jsonld", "@id": "http:///News-1545924.html", "title": "碧芯生物新品—24重可在Luminex200使用的二代羧基磁性熒光編碼微球", "pubDate": "2024-04-18T11:06:34", "upDate": "2024-04-18T11:06:35" }</script> <script language="javaScript" src="/js/JSChat.js"></script><script language="javaScript">function ChatBoxClickGXH() { DoChatBoxClickGXH('https://chat.chem17.com',538949) }</script><script>!window.jQuery && document.write('<script 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src="/skins/535403/images/nytitle_ico.png" />碧芯生物新品—24重可在Luminex200使用的二代羧基磁性熒光編碼微球</h1> <div id="miq7aop" class="detailmain"> <div id="miq7aop" class="NyNewDetail_Tips"> <span><img src="/skins/535403/images/ico04.png" />更新時間：2024-04-18</span><span><img src="/skins/535403/images/ico05.png" />點擊次數：607</span> </div> <div id="miq7aop" class="NyNewDetail_Content" id="c_detail_wrap"> <p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;">液相懸浮芯片的儀器依據光路不同，存在著兩大體系。一種基于普通流式細胞儀檢測（也稱CBA體系），分群光路為675/780 nm；一種基于Luminex100/200檢測，分群光路為658/712 nm。因為分群光路不同，對應這兩種體系的熒光編碼微球是不能兼容的，即CBA體系的微球不能用采用Luminex體系的儀器讀取，而Luminex體系的微球用CBA體系的儀器分群時也不能很好區(qū)分。</span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;"> 目前市面上的流式熒光編碼微球大多數是基于普通流式細胞儀開發(fā)的，碧芯生物也已推出可在普通流式細胞儀上使用的38重磁性熒光編碼微球（可根據要求定制更多重）。然而除了Luminex自己公司的微球（也包括BIO-RAD），市面上幾乎沒有適用于Luminex100/200的磁性熒光編碼微球。這主要Luminex公司每群圈出的微球區(qū)域熒光分布較窄，即使在兩個熒光通道的批間熒光CV高于5%都會出現(xiàn)較嚴重的偏離，在當前Luminex如此苛刻的設置下，非Luminex廠家制備能在Luminex自身的機器上使用的磁性熒光編碼微球的難度甚至比Luminex公司自身生產微球還高，需要對編碼熒光精準調控，所以迄今為止尚無任何Luminex公司以外的其他廠家能生產出直接可以在Luminex機器上使用的微球。</span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-family: 華文仿宋; visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="color:rgba(0, 0, 0, 0.9)"> 碧芯生物基于多年來在熒光編碼中積累的成熟技術，摸索了一套制備適應于Luminex200的磁性熒光編碼微球的工藝。圖1為碧芯生物</span>推出<span style="color:rgba(0, 0, 0, 0.9)">的24重可在Luminex200使用的二代羧基磁性熒光編碼微球（其它編碼可定制）。</span></span><span style="color: rgba(0, 0, 0, 0.9); margin: 0px; padding: 0px; outline: 0px; max-width: 100%; font-size: var(--articleFontsize); letter-spacing: 0.034em; font-family: 華文仿宋; visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;">這些微球的生產工藝已經成熟，可以規(guī)?；a</span><span style="color: rgba(0, 0, 0, 0.9); margin: 0px; padding: 0px; outline: 0px; max-width: 100%; letter-spacing: 0.034em; font-size: 12pt; font-family: 宋體; visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;">。</span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><img class="rich_pages wxw-img" data-imgfileid="100000565" data-ratio="0.7179190751445087" data-src="https://mmbiz.qpic.cn/sz_mmbiz_png/qfmXG4zpKiciaIibZruO03SQeRS4ZE69ZDFNlic4mgCic9zJYJhxEI2HCLVzFZ5GMlziahUJ9BlkiaFSHK4RHtOke2ndw/640?wx_fmt=png&from=appmsg" data-type="png" data-w="865" data-original-style="font-family: 宋體;font-size: 12pt;letter-spacing: 0.034em;width: 522px;height: 375px;" data-index="2" src="https://img45.chem17.com/9/20240418/638490346895283214902.jpg" _width="522px" crossorigin="anonymous" alt="碧芯生物新品—24重可在Luminex200使用的二代羧基磁性熒光編碼微球"data-fail="0" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; font-family: 宋體; font-size: 12pt; letter-spacing: 0.034em; width: 522px !important; visibility: visible !important;"/></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;">圖1 碧芯生物推出的24重可在Luminex200使用的二代羧基磁性熒光編碼微球</span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); box-sizing: border-box !important; overflow-wrap: break-word !important;"><img src="https://img41.chem17.com/9/20240418/638490348017869533709.png" alt="碧芯生物新品—24重可在Luminex200使用的二代羧基磁性熒光編碼微球"style="width:800px;"/></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;"> 碧芯生物的二代低吸附磁性微球的質量穩(wěn)定,性能超越進口<span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; letter-spacing: 0.578px; box-sizing: border-box !important; overflow-wrap: break-word !important;">Luminex</span>微球。以下是我們</span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; font-size: var(--articleFontsize); letter-spacing: 0.034em;">從兩個方面比較</span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; font-size: var(--articleFontsize); letter-spacing: 0.034em;">性能的測試結果：</span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; color: rgb(0, 0, 0);"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;">（1）非特異性吸附比較</span></span><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;">：我們用PE（藻紅蛋白）來模擬微球對蛋白的非特異性吸附。從結果中可以看出，碧芯生物的二代羧基磁性熒光編碼微球BS-6-10K-COOH- A2對PE的非特異性吸附低。</span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;">表1 碧芯生物普通羧基磁性熒光編碼微球與低吸附羧基磁性熒光編碼微球對藻紅蛋白（PE）吸附能力對比</span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; font-size: var(--articleFontsize); letter-spacing: 0.034em;"><img class="rich_pages wxw-img" data-imgfileid="100000569" data-ratio="0.4884437596302003" data-type="png" data-w="649" data-src="https://mmbiz.qpic.cn/sz_mmbiz_png/qfmXG4zpKichQKtTqWiafgCXlV2bl8u3kBichIibUhfVsqtdADrDic7OuqBhnyJUx6hCqLzdkibNLK3Rpq99TBBnG8OA/640?wx_fmt=png&from=appmsg" data-original-style="" data-index="4" src="https://img58.chem17.com/9/20240418/638490346899214710824.jpg" _width="649px" crossorigin="anonymous" alt="碧芯生物新品—24重可在Luminex200使用的二代羧基磁性熒光編碼微球"data-fail="0" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; visibility: visible !important; width: 649px !important;"/></span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;">（2）在檢測項目中的表現(xiàn)</span><strong style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;">：</span></strong><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;">偶聯(lián)抗體/抗原后用于項目檢測中熒光信號的強弱以及信噪比是衡量微球性能的最重要指標。為了驗證幾種微球在檢測項目中的表現(xiàn)，我們將這上述這幾種微球都標記好小鼠抗人VEGF抗體，并做人VEGF檢測的標準曲線。檢測信號如表1所示。從這些檢測結果來看碧芯二代羧基磁性熒光編碼微球在檢測信號方面也優(yōu)于進口的兩種微球，信號值為Luminex和Bio-Rad微球的2倍多。圖2為4種微球做出的標準曲線(五參數擬合)。</span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; font-size: var(--articleFontsize); letter-spacing: 0.034em;"><img class="rich_pages wxw-img" data-imgfileid="100000571" data-ratio="0.7294685990338164" data-type="png" data-w="1449" data-src="https://mmbiz.qpic.cn/sz_mmbiz_png/qfmXG4zpKichQKtTqWiafgCXlV2bl8u3kBCCEBibHJd8iapk4EhhjNd2PGkzpfgNFPSI4hQ5qYQ1q674wZh5ggx3MA/640?wx_fmt=png&from=appmsg" data-original-style="" data-index="5" src="https://img48.chem17.com/9/20240418/638490346901838379966.jpg" _width="677px" crossorigin="anonymous" alt="碧芯生物新品—24重可在Luminex200使用的二代羧基磁性熒光編碼微球"data-fail="0" style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; visibility: visible !important; width: 677px !important;"/></span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; text-align: center; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; font-size: var(--articleFontsize); letter-spacing: 0.034em;">圖2 <span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; letter-spacing: 0.578px; box-sizing: border-box !important; overflow-wrap: break-word !important;">五參數擬合四種熒光編碼微球做出的檢測VEGF的標準曲線。</span><br style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; letter-spacing: 0.544px; box-sizing: border-box !important; overflow-wrap: break-word !important;"></span></span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: system-ui, -apple-system, BlinkMacSystemFont, "Helvetica Neue", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei UI", "Microsoft YaHei", Arial, sans-serif; font-size: 17px; letter-spacing: 0.544px; text-align: justify; text-wrap: wrap; background-color: rgb(255, 255, 255); visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-family: 華文仿宋; visibility: visible;"> 碧芯生物的二代羧基磁性熒光微球不僅穩(wěn)定性好（包括熱穩(wěn)定性和編碼穩(wěn)定性），檢測性能*，在編碼種類上也得到了極大的豐富，可滿足各種類型儀器的需求</span></p><p style="margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; min-height: 1em; color: rgba(0, 0, 0, 0.9); 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